M.Ceolín, J.A.Martínez and M.Ermácora (1997) TDPAC of 111In-EDTA Linked to the Single Free Cysteine of Bovine Serum Albumin. J. of Biochemical and Biophysical Methods 35 135.

Time differential perturbed angular correlation of 111In-EDTA linked to the single free cysteine of bovine serum albumin.

Ceolin M, Martinez JA, Ermacora MR.

Departamento de Fisica, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.

By means of a facile chemical modification of the bovine serum albumin molecule, it was possible to measure its hydrodynamic radius with high accuracy (approximately 3%) using the TDPAC technique. The new approach presented here allows a wide use of the TDPAC technique to perform high precision studies of backbone dynamics of almost any protein.