Ronderos, J.R., Riccillo, F.L. and Ottino, A. (1996). Mosquito larvae extract induces mitotic rate alterations of mice tongue keratinocytes along a circadian rhythm period. Cell Biol Int 20(8):567-572.

Mosquito larvae extract induces mitotic rate alterations of mice tongue keratinocytes along a circadian rhythm period

Ronderos JR, Riccillo FL, Ottino A.

Catedra de Histologia y Embriologia 'B', Fac. de Cs. Medicas, Universidad Nacional de La Plata, Argentina.

It has been demonstrated that the crude extract of mosquito larvae alters the mitotic rate of several mouse cell populations of young growing mice (25 +/- 1 days old). Furthermore, the dialysed fraction of the extract inhibited proliferation of hepatocytes from hepatectomized adult male mice (90 days old). Sampling during the period between 16 and 24 h after treatment (when mitotic peak normally occurs) shows an inhibiting effect on the G1/S interphase, whereas evaluation during the dark phase of the circadian rhythm period (i.e. 4 to 12 h after treatment) shows an increment of the mitotic rates suggesting a probable effect at G2/M restriction point. In the present paper we report the effect of the mosquito larvae crude extract on the proliferative activity of tongue keratinocytes along a circadian rhythm period. Treatments were intraperitoneally applied at 16/00 Time of Day/Time post Injection and mice were killed at 00/08, 04/12, 08/16, 12/20 and 16/24 TD/TPI. As other cell populations previously analysed, the mitotic rate of tongue keratinocytes of extract receivers was significantly increased during night (when S phase normally occurs) and inhibited during the 08/16 to 16/24 period.